Human FSD1 (Fibronectin type III and SPRY domain-containing protein 1) ELISA KIT (HUFI05242)

This antibody-pair immunoassay from Assay Genie provides quantitative measurement of human Fibronectin type III and SPRY domain-containing protein 1 (FSD1) in research samples. The kit is supplied as a 96 Assays package containing a capture antibody-coated microplate, matched detection antibody, lyophilized standard calibrators, chromogenic substrate, stop solution, concentrated wash buffer, and assay diluent sufficient for one full plate run.

FSD1 is a cytoskeletal, microtubule-associated protein built around a fibronectin type III repeat paired with a SPRY/B30.2 domain, and it has appeared in published research as a candidate biomarker in non-small cell lung cancer and bladder cancer investigations. Laboratories profiling cytoskeletal organization, mitotic spindle assembly, or oncology biomarker panels apply this kit to compare FSD1 levels across cell lysates and tissue homogenates prepared from cultured human cells, with reactivity restricted to the human orthologue.

The procedure follows a standard capture-plate workflow with a chromogenic end-point read on a conventional microplate reader. The assay is intended for in vitro research use only. Reagent vials should be refrigerated on arrival; the standard and concentrated detection antibody are best aliquoted into single-use portions and stored frozen to limit freeze-thaw damage across long-term studies.