Human FRZB ELISA Kit (HUFI04235)

This sandwich-format immunoassay from Assay Genie quantifies human Frizzled-Related Protein B (FRZB, also called sFRP-3), a secreted Wnt-pathway antagonist implicated in chondrogenesis, osteoblast regulation, and tumor microenvironment studies. The kit employs human-specific capture antibodies paired with matched detection reagents to deliver quantitative readouts across replicate standards. Researchers investigating osteoarthritis, bone-density disorders, or FRZB expression in colorectal and breast cancer models commonly apply this assay to clinical research specimens. Standard workflow uses 100 uL sample additions, sequential incubation washes, and substrate development compatible with standard microplate readers. Unopened kits are stored refrigerated between uses; reconstituted reagents should be kept frozen in aliquots to limit freeze-thaw cycles. Research use only.