Human Fructosamine-3-Kinase (FN3K) ELISA Kit

Fructosamine-3-Kinase (FN3K) is a cytoplasmic kinase that initiates protein deglycation by phosphorylating fructosamines—Amadori products formed when glucose reacts non-enzymatically with protein amines. This Human FN3K ELISA Kit from Assay Genie provides a quantitative, absorbance-based immunoassay for measuring endogenous FN3K protein in human-derived samples, supporting research into diabetes, metabolic disease, and the cellular handling of glycated proteins.

The assay is supplied as a complete reagent set configured for 96 determinations in a standard microplate format. It is validated for use with plasma, tissue homogenates, and cell lysates, accommodating studies ranging from clinical specimen screening and cultured-cell experiments to tissue-based investigations of FN3K regulation. The colorimetric readout integrates with standard microplate spectrophotometers, requiring no specialized detection equipment.

Because FN3K counteracts the accumulation of advanced glycation end-products, it is of interest in studies of diabetic complications such as nephropathy, retinopathy, and vascular dysfunction, as well as in aging research and investigations of cellular stress responses. Researchers probing how glucose flux, pharmacological inhibitors, or genetic variation modulate FN3K expression can use the kit as a quantitative tool across patient cohorts, cell-line panels, and animal-model tissues with translational relevance to humans.