Human Frizzled 8 ELISA Kit (HUFI07886)

Quantitative measurement of Frizzled 8 (FZD8), a seven-transmembrane G-protein coupled receptor that binds Wnt ligands, is enabled by this immunoassay kit from Assay Genie. The kit is configured for research use only and is intended to support investigations into canonical Wnt/β-catenin signaling and non-canonical Wnt pathways.

Frizzled receptors play central roles in embryonic development, stem cell maintenance, and tissue homeostasis, and altered FZD8 expression has been documented in oncology research, including studies of triple-negative breast cancer, colorectal tumors, and gastric cancer cell lines. Researchers apply this reagent to track receptor abundance across differentiation protocols, screen for Wnt-pathway modulation by small molecules or biologics, and validate transcriptomic findings that flag FZD8 as a differentially expressed target.

The immunoassay procedure works with cell culture supernatants, cell lysates, and tissue-derived research samples. Capture and detection reagents, standards, and buffers are supplied to complete the run, and components are stored refrigerated between uses. The plate-based workflow allows parallel processing of experimental and control conditions within the same run.

Beyond signaling pathway studies, the kit is used in cancer biology programs focused on tumor-microenvironment interactions, in regenerative medicine research where Wnt-driven β-catenin activity guides lineage decisions, and in neuroscience investigations of Frizzled family function. Assay Genie provides the supporting protocol documentation required for routine laboratory implementation.