Human FPGS (Folylpolyglutamate synthase) ELISA Kit (HUFI04747)

Beyond the validated analytical performance already described, the Assay Genie FPGS kit runs as a 4 hour chromogenic reaction (excluding equilibration and sample preparation), and the pre-coated microplate accepts a sample volume of 50 uL per well for serum or plasma and 100 uL per well for cell culture supernatant or tissue lysate. The biotin-streptavidin-HRP amplification chemistry delivers intra-assay precision reported below 8 percent CV and inter-assay precision below 10 percent CV, while spike-and-recovery studies in human serum, EDTA plasma, and heparin plasma fall within roughly 85 to 105 percent of expected values, indicating low matrix interference. Antibody specificity has been characterized with no obvious cross-reactivity toward analogous folate-handling enzymes, an important control when profiling antifolate-resistant sublines in which paralog expression may shift. The kit is supplied in either a 48-assay or 96-assay configuration, with sealed components that include TMB substrate, 25X wash buffer, SABC dilution buffer, antibody dilution buffer, and stop solution; opened reagents are held at 2 to 8 degrees C and must not be frozen per the manufacturer's stability data.