Human FOXP3/Forkhead box protein P3 ELISA Kit

A high-quality sandwich ELISA from Assay Genie designed for the quantitative detection of human Forkhead box protein P3 (FOXP3), a key regulatory transcription factor involved in the development and function of regulatory T cells. The assay uses a double-antibody sandwich format with colorimetric detection and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples.

Reported performance characteristics include a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Spike-and-recovery across matrices averages 93% for serum (range 86–104%), 91% for EDTA plasma (range 87–96%), and 95% for heparin plasma (range 91–102%). Parallelism on serial dilutions (1:2, 1:4, 1:8) is reported at 83–99% / 83–98% / 88–97% for serum, 91–97% / 84–100% / 83–98% for EDTA plasma, and 81–98% / 85–99% / 81–96% for heparin plasma. The kit is stored at 2–8°C with a 12-month shelf life.

FOXP3 is a transcriptional regulator required for the suppressive function and lineage stability of regulatory T cells. Dysregulation of FOXP3 has been implicated in autoimmune diseases, inflammatory disorders, and cancer, supporting its use as a biomarker in studies of immune regulation, autoimmunity, and cancer biology.