Human FOXO3/Forkhead box protein O3 ELISA Kit

Assay Genie provides a Sandwich ELISA kit designed for the precise measurement of FOXO3 (Forkhead Box Protein O3) levels in human samples. Using a double antibody sandwich format with colorimetric detection, the kit is intended for research applications and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay reports a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL, with kit storage at 2-8°C for 12 months.

FOXO3 is a key transcription factor that regulates genes involved in apoptosis, cell cycle arrest, and oxidative stress response. Its dysregulation has been implicated in cancer, diabetes, and aging-related disorders, making it a potential therapeutic target and biomarker for disease progression. The kit is engineered to deliver robust, consistent, and reproducible results, supporting research into FOXO3's role across physiological and pathological conditions.

Validated recovery rates for serum (n=5) range from 86-105% with an average of 96%, EDTA plasma (n=5) 88-105% averaging 95%, and heparin plasma (n=5) 88-104% averaging 95%. Linearity data across dilutions 1:2, 1:4, and 1:8 demonstrate assay performance in serum, EDTA plasma, and heparin plasma matrices.