Human FoxO3/FKHRL1 PharmaGenie ELISA Kit (SBRS0597)

FoxO3 (also called FKHRL1) is a forkhead-box transcription factor that regulates apoptosis, cell-cycle arrest, oxidative-stress responses, and autophagy pathways, making it a frequent target in oncology, aging, and metabolism research. This quantitative immunoassay from Assay Genie provides a colorimetric readout of human FoxO3/FKHRL1 protein concentrations in cell culture supernatants, plasma, and serum samples. Each kit supplies pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, standards, and buffers sufficient for 96 assays, supporting in vitro workflows that demand reproducible measurement across many biological replicates.

The kit is intended for research use only and is compatible with standard microplate readers capable of measuring absorbance in the visible range. Researchers studying PI3K/AKT signaling, caloric restriction, neurodegeneration, or tumor suppression often rely on FoxO3 protein quantification alongside gene-expression analyses to connect transcriptional activity with downstream phenotypes. The assay's three-sample-type flexibility allows direct comparison of circulating FoxO3 levels in clinical plasma or serum specimens with in vitro cell-culture models, reducing the need to switch between separate assays.