Human Forkhead box protein O1 (FOXO1) ELISA Kit

Manufactured by Assay Genie, the Human Forkhead box protein O1 (FOXO1) ELISA Kit is a Sandwich-format immunoassay for the quantitative detection of human FOXO1 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit uses an ELISA detection method and is supplied for research use only.

Reported performance figures include a detection range of 0.156-10ng/mL and a sensitivity of 0.078ng/mL. Intra-assay CV is 4.5% and inter-assay CV is 7.0%. Linearity for serum (N=5) spans 86-97% at 1:2 to 108-117% at 1:16; EDTA plasma spans 105-116% at 1:2 to 87-96% at 1:16; heparin plasma spans 91-101% at 1:2 to 88-100% at 1:16. Recovery averages 102% (range 96-108%) in serum and 104% (range 98-110%) in plasma.

FOXO1 is a transcription factor that acts as the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. The protein is catalogued under UniProt entry Q12778 and is localised in the cytoplasm and nucleus. The assay detects natural and recombinant human FOXO1 and is associated with the cancer research area. Also referred to as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FKHR and FOXO1A, the protein participates in cell cycle regulation, apoptosis, metabolism and oxidative stress response, and its dysregulation has been linked to cancer, diabetes and neurodegenerative disorders.