Human FNTA ELISA Kit (HUFI04259)

The Assay Genie FNTA immunoassay provides researchers with a quantitative tool for measuring farnesyltransferase alpha subunit levels in human specimens. FNTA encodes the alpha subunit of CAAX farnesyltransferase, a cytosolic enzyme that catalyzes the first committed step in protein prenylation. The enzyme transfers a farnesyl group from farnesyl pyrophosphate onto the cysteine residue of the C-terminal CAAX motif found on substrate proteins such as Ras GTPases, Rho family members, and nuclear lamins. This lipid modification anchors substrate proteins to intracellular membranes and is essential for downstream signaling that governs cell proliferation, differentiation, and survival.

Quantitative measurement of FNTA protein supports research into prenylation-dependent oncogenic pathways, since aberrant farnesylation of Ras contributes to tumor development in many cancers. Pharmacological farnesyltransferase inhibitors are studied as targeted anticancer agents, and accurate quantification of FNTA levels aids in evaluating inhibitor efficacy, enzyme expression under drug treatment, and substrate selectivity studies. The immunoassay also serves researchers exploring congenital disorders linked to disrupted prenylation, age-related changes in farnesylation capacity, and cross-talk between farnesylation and geranylgeranylation pathways in cell biology and signal transduction studies.

Assay Genie supplies the FNTA detection kit as a complete package containing a pre-coated microplate, calibrated recombinant standards, biotinylated detection antibody, streptavidin conjugate, wash buffer, and substrate solution. Each kit provides reagents for processing samples in the standard plate format, and the protocol is compatible with conventional microplate absorbance readers used in most research laboratories investigating post-translational lipid modification.