Human FNBP1L (Formin-binding protein 1-like) ELISA KIT (HUFI05219)

Quantifies formin-binding protein 1-like (FNBP1L), a multifunctional scaffold protein also known as TOCA-1 (Transducer of Cdc42-dependent actin assembly). FNBP1L contains an F-BAR domain that binds curved membranes and recruits N-WASP to drive Cdc42-mediated actin polymerization, with documented roles in membrane tubulation, endocytosis, invadopodia formation, and cell migration.

This immunoassay from Assay Genie supports research into cancer cell invasion, neural development, and cytoskeletal remodeling, where FNBP1L expression patterns may indicate how cells coordinate membrane dynamics with the actin cytoskeleton. Plasma-derived specimens from human donors are compatible with the workflow, which yields an absorbance-based read-out across a standard curve.

Each package provides reagents sufficient for 96 individual determinations in a microwell plate format. The procedure follows standard immunoassay practice: pre-coated wells, sample addition, sequential incubation with detection reagents, wash steps, and substrate development. Researchers should consult the product insert for parameters including recommended dilution factors, incubation times, and expected values for their biological system.

Store unopened kits under refrigerated conditions, with stability maintained through the printed expiration date. Working-dilution reagents should be handled according to the package insert to preserve activity across repeated freeze-thaw cycles. For research use only; not for diagnostic procedures.