Human FLN alpha (Filamin A, Alpha) ELISA Kit (HUES03004)

The Assay Genie Human FLN alpha ELISA Kit is built around a sandwich immunoassay workflow on a pre-coated 96-well microplate, with colorimetric detection read on a standard plate reader at 450 nm. It is calibrated for quantitative measurement of Filamin A in serum, plasma, and other biological fluids, supporting research into cytoskeletal remodeling, mechanotransduction, and cell migration pathways where FLNA functions as an actin cross-linking scaffold protein.

Filamin A (also referred to as FLNA or ABP-280) is a large ~280 kDa actin-binding protein that organizes the cortical actin cytoskeleton and anchors transmembrane receptors, integrins, and signaling intermediates to the underlying cytoskeleton. Studies have linked FLNA dysregulation to periventricular heterotopia, frontometaphyseal dysplasia, otopalatodigital syndrome, and several carcinomas, making quantitative plasma profiling useful in neurology, developmental biology, and oncology research workflows.

For assay setup, store the kit refrigerated at 2-8 °C, avoid repeated freeze-thaw cycles of reagents, and equilibrate all components to room temperature before running the protocol. The 96-well plate format, in vitro research-use-only designation, and human-specific reactivity make this microplate kit a practical option for biomarker studies, comparative profiling of patient cohorts, and validation of FLNA changes alongside western blot or PCR data.