Human FLJ25006 ELISA Kit (HUFI04268)

The FLJ25006 protein, encoded by the C16orf71 gene on human chromosome 16, is an uncharacterized open reading frame of interest in expression profiling and biomarker discovery research. This Assay Genie immunoassay kit provides quantitative measurement of human FLJ25006 in research specimens, supporting studies in proteomics, target validation, and translational research workflows.

Each microplate-based kit includes pre-coated wells, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. The standard curve spans recombinant FLJ25006 calibrators, allowing interpolation of unknown sample concentrations against the reference curve. A 50 μL sample volume per well is recommended, with standards and unknowns run in duplicate for reliable interpolation.

For optimal reagent stability, store unopened components at -20 degrees C until first use; avoid repeated freeze-thaw cycles by aliquoting working solutions. Bring all reagents to room temperature before running the assay. Allow freshly collected samples to clot completely before serum preparation, and process plasma with appropriate anticoagulants per standard venipuncture protocols.

Common research applications include protein expression analysis across tissue types, investigation of disease-associated dysregulation in patient cohorts, and validation of transcriptomic findings at the protein level. The kit is intended for research use only and is not validated for diagnostic procedures.