Human FLAD1 (FAD synthase) ELISA KIT (HUFI05243)

This Assay Genie ELISA kit supports quantitative measurement of FAD synthase in human research samples, useful for studies of mitochondrial flavin metabolism and inherited metabolic disorders. FLAD1 encodes the bifunctional enzyme responsible for the phosphorylation of riboflavin to FMN and the subsequent adenylation to FAD, completing flavin cofactor biosynthesis. Researchers investigating multiple acyl-CoA dehydrogenase deficiency, lipid storage myopathy, and riboflavin-responsive neuromuscular phenotypes use this assay to track enzyme expression in patient cohorts and cell models. The kit is supplied with a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution, with a recommended sample volume of 100 uL per well. Aliquot the reconstituted standard after first use to limit repeated freeze-thaw, and keep kit components refrigerated between runs to preserve activity. The colorimetric read-out is compatible with standard microplate readers in research workflows.