Human FKBP6 ELISA Kit (HUFI04262)

Quantitative profiling of FK506-binding protein 6 (FKBP6) for reproductive biology and immunophilin signaling research is the focus of the Assay Genie ELISA Kit, which uses paired antibodies against distinct human epitopes of this testis-enriched protein. FKBP6 maps to chromosome 7q11.23 within the Williams-Beuren syndrome critical region, and loss-of-function variants have been tied to non-obstructive male factor infertility in published cohorts, which is why reproductive and germ-cell laboratories track it as a translational biomarker.

The assay generates a chromogenic read-out measurable on a standard laboratory microplate reader. Workflows supported by the kit include characterizing cell culture systems under immunosuppressant challenge, comparing patient versus control cohorts in male-fertility studies, and confirming knock-down or knock-out efficiency in functional genomics screens. Each pack is supplied in a 96 Assays configuration containing a pre-coated plate, calibration standards, detection reagent, wash concentrate, and substrate, and is intended for research use only.

For best reproducibility, aliquot serum or plasma samples on receipt and freeze them at -80 degrees C, limiting freeze-thaw exposure so the target protein does not degrade. The kit is shipped refrigerated on blue ice to preserve its calibration curves. Lot-specific validation data and a step-by-step protocol are bundled with every pack, allowing users to document their own standard curves on the plate reader available in their laboratory.