Human FKBP10 ELISA Kit (HUFI04274)

The Assay Genie Human FKBP10 ELISA Kit is a quantitative immunoassay for measuring FKBP10 protein levels in human research specimens. FKBP10 encodes a 65 kDa peptidyl-prolyl cis-trans isomerase localized to the endoplasmic reticulum, where it functions as a collagen chaperone that supports proper folding of type I procollagen chains and hydroxylation of proline residues.

This assay enables investigations into connective tissue biology, with particular relevance for studies of Bruck syndrome and Osteogenesis Imperfecta type XI, where loss-of-function mutations in FKBP10 disrupt collagen maturation. The kit utilizes a well plate configuration compatible with standard plate readers, allowing medium-throughput analysis of clinical samples. For optimal stability, researchers should aliquot reagents upon receipt, store kit components at refrigerated temperatures, and minimize freeze-thaw cycles to preserve antibody binding activity.

Validated as an in vitro research use only reagent, the kit supports reproducible quantification of FKBP10 abundance across cell lysates, conditioned media, and patient-derived specimens. Common applications include profiling FKBP10 expression in dermal fibroblasts, correlating protein levels with collagen maturation markers, and screening therapeutic compounds that modulate prolyl hydroxylation pathways relevant to rare skeletal disorder research.