Human Ficolin-3 PharmaGenie ELISA Kit (SBRS0562)

Ficolin-3 is a pattern recognition molecule of the lectin complement pathway and a soluble effector of innate immunity. Secreted primarily by hepatocytes into the bloodstream, it circulates as oligomers that bind acetyl groups on microbial surfaces, triggering complement activation through MASP-associated serine proteases. Research on circulating Ficolin-3 has linked altered serum concentrations to recurrent infections, autoimmune disease, and certain cancer phenotypes, making quantitative measurement valuable in immunology and biomarker studies.

The Assay Genie Human Ficolin-3 PharmaGenie ELISA Kit is configured as a complete quantitative immunoassay supplied as a microplate-based kit covering 96 determinations. The assay uses a two-antibody capture format with matched antibody pairs specific to human Ficolin-3. Each kit contains a pre-coated microplate, lyophilized standards, detection antibody, assay diluent, wash buffer, substrate, and stop solution, packaged in individual vials. The procedure follows a standard incubation-and-wash protocol with a colorimetric endpoint measured on a standard plate reader.

Intended to quantify human Ficolin-3 in serum samples, plasma samples, and cell culture supernatants, the assay supports research use only in immunology, complement biology, infectious disease, oncology, and biomarker discovery. Components are intended for refrigerated storage, and the kit is supplied as a single-use set of reagents sufficient for 96 assays under standard laboratory conditions.