Human Ficolin-2 PharmaGenie ELISA Kit (SBRS0561)

A research-use ELISA kit from Assay Genie enables quantification of Ficolin-2 (FCN2), a liver-derived pattern recognition protein that circulates in human blood and triggers the lectin complement pathway during innate immune responses. The assay accepts samples such as cell culture supernatants and blood fractions collected as serum or plasma, producing an absorbance-based readout on a standard microplate reader. Each kit is supplied with pre-coated plate wells, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant protein standards, wash buffer, and substrate solution sufficient for a full plate run.

Investigators studying lectin pathway deficiencies, autoimmune conditions, recurrent infections, or chronic inflammation use this kit to measure circulating Ficolin-2 concentrations as an indicator of complement activation status. The workflow follows standard plate-based immunoassay steps: addition of diluted samples and standards, incubation, washing, detection reagent binding, chromogenic substrate development, and absorbance measurement at an appropriate wavelength.

Kit components should be refrigerated and protected from light to preserve reagent activity through the stated shelf life. Refer to the enclosed protocol for recommended dilution factors, incubation times, and temperature settings before beginning the assay. The PharmaGenie reagent line from Assay Genie supports immunology, complement, and biomarker research workflows.