Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of human Fibronectin type III domain-containing protein 5 (FNDC5) in human samples. The kit, which is for research use only, recognises natural and recombinant human FNDC5, also known by the synonyms Fibronectin type III repeat-containing protein 2 and FRCP2 (UniProt Q8NAU1). It is intended for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and is positioned within the metabolism research area, where FNDC5-derived irisin is studied in the context of muscular exercise and adipose tissue biology.

Manufacturer-reported performance figures include a detection range of 0.78-50ng/ml and a sensitivity of 0.12ng/mL. Intra-assay precision is 4.3% (CV) and inter-assay precision is 8.5% (CV). Linearity across dilutions 1:2, 1:4, 1:8 and 1:16 is reported as 97-106%, 99-109%, 85-95% and 92-104% for serum; 109-118%, 101-111%, 107-117% and 94-103% for EDTA plasma; and 107-116%, 90-102%, 80-89% and 91-103% for heparin plasma. Spike-and-recovery averages 90% (range 84-96%) in serum and 92% (range 86-98%) in plasma.