Human Fibronectin type III domain-containing protein 3B (FNDC3B) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise detection of human Fibronectin type III domain-containing protein 3B (FNDC3B) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Using a colourimetric detection method, the assay has a detection range of 0.625-40ng/mL and a sensitivity of 0.336ng/mL, and is specific to natural and recombinant human FNDC3B.

FNDC3B contains fibronectin type III domains involved in cell adhesion and migration, and is annotated as a possible positive regulator of adipogenesis. Dysregulation of the protein has been linked to cancer and developmental disorders, so reliable quantification supports research into disease mechanisms and potential targeted therapies. The kit is supplied with a dismountable 8×12-strip microplate, lyophilised standard, sample and assay diluents, detection reagents A and B, wash buffer, substrate solution, stop solution and plate sealers, and is intended for research use only.

Reported precision includes an intra-assay CV of 6.9% and inter-assay CV of 8.2%. Recovery averages 86% (range 80-92%) for serum and 88% (range 82-94%) for plasma. Linearity across 1:2 to 1:16 dilutions spans 82-118% for serum, 93-112% for EDTA plasma and 82-117% for heparin plasma. The target is a single-pass membrane protein (UniProt Q53EP0) and the research area is signal transduction.