Human Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit

Quantify human FGF1 in research specimens with this competitive-format immunoassay from Assay Genie. The kit targets the acidic isoform of fibroblast growth factor, also known as HBGF-1 or aFGF, an approximately 17 kDa mitogenic signaling protein implicated in angiogenesis, embryonic development, tissue repair, neurogenesis, and metabolic regulation.

FGF1 binds to FGFR1-4 receptors and has been studied extensively in cancer biology, stem cell maintenance, and regenerative medicine contexts. The assay is configured for 96 determinations in a microplate layout and works with multiple input matrices, including cell lysates, tissue homogenate fractions, and cell culture supernatant samples.

All reagents required to run the assay, including coated wells, detection antibody, standards, and substrate solution, are supplied ready for use. The protocol produces a quantifiable signal proportional to FGF1 concentration in the sample, enabling researchers to compare experimental conditions, monitor expression changes in cell-based models, or screen specimens for FGF1 content.