Human Fibrinogen ELISA Kit

Designed for the quantitative measurement of fibrinogen in human samples, this sandwich ELISA kit from Assay Genie uses a double antibody format with colorimetric detection. Validated sample types include plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The workflow follows a six-step protocol ending with TMB substrate development and absorbance reading at 450 nm, and the kit is intended to be stored at 2-8°C for 12 months.

According to the manufacturer's specifications, sensitivity is 0.938 ng/mL with an assay range of 1.563-100 ng/mL, and the kit is positioned for research into fibrinogen's role in blood clot formation and hemostasis. The kit is described as a tool for studying abnormal fibrinogen levels, which have been linked to cardiovascular disease, stroke, and inflammatory disorders. Reported linearity for EDTA plasma (n=5) is 81-94% at 1:4 and 89-104% at 1:8, and for heparin plasma (n=5) 86-99% at 1:2, 85-95% at 1:4, and 82-95% at 1:8. Recovery averages 91% for EDTA plasma (n=5, range 87-105%) and 94% for heparin plasma (n=5, range 85-98%).