Human Fibrillin-1 (FBN1) ELISA Kit

Quantify circulating human fibrillin-1 with this immunoassay kit built for connective tissue and matrix biology research. Fibrillin-1 is a large extracellular glycoprotein that forms the backbone of microfibrils, structures that confer elastic recoil to connective tissues and act as scaffolds for elastic fiber assembly. Mutations and dysregulation of the FBN1 gene have been linked to Marfan syndrome, stiff skin syndrome, and a spectrum of heritable connective tissue disorders, so reliable measurement of fibrillin-1 in circulation supports cardiovascular research, dermatology studies, and investigations of age-related matrix remodeling.

Assay Genie supplies the kit as a complete reagent set with a pre-coated microplate, biotinylated detection reagent, recombinant protein standard, and the buffers and chromogenic substrate required to develop the assay. Researchers apply the components to research specimens, generate a standard curve, and interpolate endogenous fibrillin-1 concentrations from the optical signal. Store the unopened kit refrigerated in the dark and consult the included manual for recommended sample dilutions, assay procedure, and performance characteristics.

Typical applications include biomarker discovery in Marfan-related cohorts, monitoring of fibrillin remodeling during wound healing, and characterization of extracellular matrix turnover in cell culture models. Pair the plate readout with western blot, immunohistochemistry, or PCR experiments to map matrix changes at the protein and transcript level.