Human FGL2/Fibrinogen Like Protein 2 ELISA Kit

A high quality Sandwich ELISA Kit for the precise detection of FGL2 (Fibrinogen-Like Protein 2) levels in human samples, manufactured by Assay Genie. The kit uses a double antibody sandwich format with colorimetric detection and is suited to research into immune regulation and inflammation, with relevance to cancer, autoimmune disorders, and viral infections. Synonyms for the target protein include FGL2, Fibroleukin, and T49, and the research area is cell biology.

The assay delivers a sensitivity of 28.125 pg/mL and a standard curve range of 46.875-3000 pg/mL. Validated sample types include plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Unopened kits should be stored at 2-8°C for up to 12 months. Linearity in EDTA plasma (n=5) is 85-95% at a 1:4 dilution and 83-100% at 1:8, while heparin plasma (n=5) yields 83-98% (1:2), 82-94% (1:4), and 80-100% (1:8). Recovery ranges are 88-100% (average 96%) for EDTA plasma and 86-105% (average 94%) for heparin plasma.

The protocol involves equilibrating reagents and TMB substrate to room temperature, loading standards and samples for a 90-minute primary incubation at 37°C, adding biotin-labeled detection antibody for 60 minutes, applying HRP-streptavidin (SABC) for 30 minutes, developing color with TMB substrate for 10-20 minutes in the dark, and reading absorbance at 450 nm immediately after adding stop solution.