Human FGFR2/CD332/FGF Receptor 2 ELISA Kit

A Sandwich ELISA from Assay Genie is designed for the colorimetric, quantitative measurement of human FGF Receptor 2 (FGFR2, also known as CD332). The double-antibody format supports research into cell growth, differentiation, and development, with the manufacturer noting links between FGFR2 dysregulation and cancer, skeletal disorders, and craniosynostosis (S1).

The kit is validated for human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples (S1). Reported sensitivity is 18.75 pg/mL with a working range of 31.25–2000 pg/mL (S1). Linearity across 1:2, 1:4, and 1:8 dilutions: serum 88–92%, 87–100%, 91–101%; EDTA plasma 84–100%, 82–99%, 84–100%; heparin plasma 82–100%, 88–100%, 80–91% (S1). Recovery ranges are 95–105% for serum (average 99%), 86–100% for EDTA plasma (average 95%), and 86–105% for heparin plasma (average 94%) (S1).

The protocol includes a 90-minute primary incubation at 37°C, 60-minute detection antibody binding, 30-minute HRP-streptavidin step, and 10–20 minute TMB color development with absorbance read at 450 nm (S1). Kits are stored at 2–8°C for up to 12 months (S1). Database identifiers include KEGG K05093, UniProt P21802, and Gene ID 2263 (S2, S3).