Human FGFBP2 (Fibroblast Growth Factor Binding Protein 2) ELISA Kit (HUES02210)

Designed by Assay Genie for research use, this Sandwich ELISA Kit enables the quantitative measurement of human Fibroblast Growth Factor Binding Protein 2 (FGFBP2) in serum, plasma, and other biological fluids. The assay follows a Sandwich-ELISA format with a colorimetric detection method. Samples bind to a pre-coated capture antibody, followed by incubation with a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate, with optical density read at 450 nm ± 2 nm.

The kit shows a sensitivity of 0.94 ng/mL and a detection range of 1.56-100 ng/mL, with a stated recovery of 80%-120%. The recommended sample volume is 100 μL. It specifically recognizes human FGFBP2, and no significant cross-reactivity or interference between FGFBP2 and analogues was observed. Intra- and inter-assay precision CV values range from approximately 4.04% to 8.49% based on the manufacturer's data.

The total assay time is 3 h 30 min, with kit components stored at 2-8°C. The associated UniProt ID is Q9BYJ0, and reported synonyms for the target include FGF-BP2, HBP17RP, and KSP37. The kit is intended for signal transduction research.