Human FGF22 (Fibroblast growth factor 22) ELISA Kit (HUFI08180)

Manufactured by Assay Genie, this sandwich ELISA kit uses a double-antibody, colorimetric format for the in vitro quantitative determination of human FGF22 (Fibroblast growth factor 22) concentrations in serum, plasma, cell culture supernatants, and other biological fluids. The kit is reactive with human samples and is intended for research use only.

Performance specifications include a sensitivity of < 9.375pg/ml and an assay range of 15.625-1000pg/ml. Spike-and-recovery was assessed across matrices, with serum (n=5) yielding a recovery range of 90-105% (average 99%), EDTA plasma (n=5) 86-100% (average 92%), and heparin plasma (n=5) 87-99% (average 91%). Linearity was demonstrated by serial dilution of spiked samples: serum ranged 85-103%, 88-104%, and 86-102% at 1:2, 1:4, and 1:8; EDTA plasma ranged 86-100%, 84-99%, and 83-99%; and UFH plasma ranged 82-98%, 88-100%, and 80-100%. Precision is reported as intra-assay CV<8% and inter-assay CV<10%. The kit is stored at 4°C for 6 months.

According to UniProt, FGF22 plays a role in the fasting response, glucose homeostasis, lipolysis and lipogenesis, can stimulate cell proliferation in vitro, and may be involved in hair development; it belongs to the heparin-binding growth factors family. NCBI describes FGF22 as a member of the fibroblast growth factor family with broad mitogenic and cell survival activities, involved in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.