Human FGD5 ELISA Kit (HUFI04288)

Quantify FGD5, a Rho family guanine nucleotide exchange factor implicated in angiogenic signaling and endothelial cell behavior, with this in vitro research-use ELISA from Assay Genie. The microplate-based kit provides 96 assays configured for measurement of FGD5 in serum-derived and plasma-derived human specimens using an absorbance readout, supporting studies in vascular biology, endothelial function, and biomarker discovery where altered FGD5 expression has been reported in conditions such as pre-eclampsia.

Reagents are supplied ready to use or lyophilized for reconstitution with the kit diluent, and the procedure follows a capture-and-detection antibody format in which the coated plate immobilizes the target analyte for subsequent detection. Researchers working on VEGF pathway investigations, hypertension-associated vascular remodeling, or placental angiogenesis can incorporate the assay into existing workflows without specialized equipment beyond a standard plate reader.

Each kit includes a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate solution, and stop reagent. Handle components per the enclosed protocol: refrigerate the kit upon arrival, allow plates and reagents to equilibrate to room temperature before running the assay, and avoid repeated freeze-thaw cycles of reconstituted standards to preserve binding activity.