Human FGA/Fibrinogen Alpha ELISA Kit

Assay Genie provides a Sandwich ELISA with double-antibody configuration for the quantitative measurement of Fibrinogen Alpha (FGA) in human specimens. The assay uses a colorimetric readout and accepts plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Fibrinogen Alpha is described as a key component of the blood clotting process and is implicated in cardiovascular disorders and inflammatory conditions, supporting research into disease mechanisms and potential treatment strategies.

Performance characteristics quoted from the manufacturer include a sensitivity of 0.281 ng/mL and an assay range of 0.469-30 ng/mL, with components intended for storage at 2-8°C for 12 months. Validation in EDTA plasma (n=5) gave a recovery range of 86-101% (average 90%) and in heparin plasma (n=5) a recovery range of 87-103% (average 93%). Linearity in EDTA plasma (n=5) was 86-90% at 1:4 dilution and 82-100% at 1:8; in heparin plasma (n=5) it was 81-100% at 1:2, 90-98% at 1:4, and 82-98% at 1:8. The protocol involves equilibrating reagents, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-streptavidin binding at 37°C for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution addition.

Sample preparation guidance covers serum (clot then centrifuge at 1000 × g for 20 minutes), plasma (anticoagulant collection then centrifuge at 1000 × g for 15 minutes at 2-8°C), tissue homogenate (PBS with protease inhibitors), cell culture supernatant (centrifuge at 2500 rpm for 5 minutes), and cell lysate (lysis buffer with protease inhibitors). Associated identifiers include Uniprot P02671 and a research-area classification of Immunology.