Human FG beta (Fibrinogen Beta) CLIA Kit (HUES01127)

A chemiluminescence immunoassay (CLIA) kit from Assay Genie, intended for the in vitro quantitative determination of Human FG beta (Fibrinogen Beta) concentrations in serum, plasma and other biological fluids. The kit is reactive against Human FGβ, and the manufacturer states that no significant cross-reactivity or interference between Human FGβ and analogues was observed. The listed research area is Immunology.

The assay follows the Sandwich-CLIA principle on a pre-coated micro CLIA plate, using a biotinylated detection antibody and an Avidin-HRP conjugate. Signal is read as a relative light unit (RLU) on a chemiluminescence immunoassay analyzer, with the RLU value positively associated with Human FGβ concentration. The stated assay time is 3.5h, sensitivity is 0.19 ng/mL, and the detection range is 0.31-20 ng/mL. The required sample volume is 100μL, and the manufacturer reports reproducibility with both intra-CV and inter-CV < 15%. The format is solid phase.

Kit components include a CLIA plate, reference standard, concentrated biotinylated detection antibody (100×), concentrated HRP conjugate (100×), reference standard and sample diluent, biotinylated detection antibody diluent, HRP conjugate diluent, concentrated wash buffer (25×), substrate reagents A and B, desiccant, plate sealer, and a product description.