Human Fetuin A/AHSG ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the precise quantification of fetuin-A (AHSG) levels in human samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Using a double antibody detection method, the kit offers a sensitivity of 0.469 ng/mL and a measurable range of 0.781–50 ng/mL, making it well-suited for a variety of research applications. Fetuin-A, also known by synonyms such as A2HS, AHS, FETUA, alpha-2-HS-glycoprotein, and alpha-2-Z-globulin, is a multifunctional glycoprotein implicated in inflammation, insulin resistance, and bone metabolism, and is studied as a biomarker in diabetes, cardiovascular disorders, and liver conditions.

The kit is intended for use with human samples and should be stored at 2–8°C for up to 12 months. Validation data includes linearity and recovery performance across serum, EDTA plasma, and heparin plasma samples. The protocol follows a standard sandwich ELISA workflow with steps for reagent equilibration, primary incubation, biotin-labeled detection antibody binding, HRP-streptavidin binding, TMB color development, and absorbance measurement at 450 nm.