Human FETUB/Fetuin B ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of human Fetuin B (FETUB) by colorimetric measurement. It is intended for use with human serum and plasma samples, and the manufacturer lists additional compatible sample types including cell culture supernatant, cell or tissue lysate, and other liquid samples.

The assay is configured as a double-antibody Sandwich ELISA with a stated sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. Manufacturer performance data report serum recovery of 86–104% (average 96%), EDTA plasma recovery of 88–96% (average 94%), and heparin plasma recovery of 86–103% (average 98%). Reported linearity ranges are 83–105% for serum, 81–105% for EDTA plasma, and 81–101% for heparin plasma across tested dilutions. Fetuin B is described in the accompanying literature as a protein implicated in insulin resistance, lipid metabolism, and inflammation, with dysregulation associated with metabolic disorders.

The assay uses a biotin-labeled detection antibody and HRP-streptavidin binding, with TMB as the chromogenic substrate and absorbance read at 450 nm. Reagents are intended to be stored at 2-8°C for 12 months. The product is identified with Uniprot Q9UGM5 and is classified for research within the developmental biology field.