Human Ferritin heavy chain ELISA Kit

Offered by Assay Genie, this Sandwich ELISA kit is designed for the precise quantitative measurement of ferritin heavy chain levels in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Ferritin is a key protein responsible for iron storage and regulation in the body, playing a vital role in maintaining iron homeostasis. Abnormal ferritin levels have been linked to various health conditions, including iron deficiency anemia, hemochromatosis, and inflammatory disorders, making this protein a critical biomarker for studying such diseases and developing targeted treatments.

The assay uses a colorimetric detection method and has a sensitivity of 46.875 pg/mL with a measuring range of 78.125–5000 pg/mL. The kit is reactive with human samples and is intended for cell biology research. It targets the ferritin heavy chain (UniProt P02794), with associated synonyms including FTH1, FHC, FTH, PLIF, FTHL6, PIG15, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, and Ferritin, Heavy Polypeptide. The kit should be stored at 2–8°C for 12 months.

Validated linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma across serial dilutions. The assay procedure follows a six-step workflow: reagent and plate preparation with incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin incubation for 30 minutes, TMB substrate color development in the dark for 10–20 minutes, and a stop reaction with absorbance read at 450 nm. Sample preparation guidance covers serum, plasma, tissue homogenates, cell culture supernatants, and cell lysates.