Human FDX1 ELISA Kit (HUFI04296)

Ferredoxin 1 (FDX1) is a mitochondrial iron-sulfur cluster scaffold protein that has emerged as a central regulator of copper-induced cell death (cuproptosis) in recent cancer-metabolism research. The ELISA kit from Assay Genie provides quantitative measurement of human FDX1 in research specimens, supporting investigations into cuproptosis sensitivity, mitochondrial iron-sulfur biogenesis, and the cellular response to copper-ionophore drugs such as elesclomol. The kit is supplied as a complete package containing pre-coated microwell strips, biotinylated detection reagents, streptavidin-enzyme conjugate, calibrated protein reference standards, wash buffer, chromogenic substrate, and stop solution, configured for one full plate run of 96 Assays per kit. Researchers apply this assay to profile FDX1 levels in matched tumour and adjacent normal tissue lysates, in patient-derived plasma, and in copper-treated cell-line models where FDX1 status has been linked to lipoylated TCA-cycle enzyme integrity. The workflow includes standard-curve preparation by serial dilution, sequential incubation of samples and detection antibody in the coated wells, washing, substrate development, and absorbance measurement to interpolate FDX1 concentration from the reference curve. Reconstituted reagents remain stable for short-term refrigerated use; repeated freeze-thaw cycles of the standards should be avoided. The product is supplied for research use only and is not intended for clinical diagnostic applications.