Human FDP/Fibrinogen Degradation Product ELISA Kit

Quantitative measurement of fibrinogen degradation products in human specimens is central to evaluating coagulopathies, disseminated intravascular coagulation, deep vein thrombosis, and pulmonary embolism. The Assay Genie microplate immunoassay provides a 96-well sandwich ELISA workflow with colorimetric readout for research use only, delivering results through a standard curve generated from calibrators supplied with the kit. Each box contains a pre-coated microplate, biotinylated detection antibody, lyophilized standards, assay buffers, and TMB substrate sufficient for 96 individual determinations.

Sensitivity is rated below 2.5 ng/mL, with a standard curve detection range of 6.25 to 400 ng/mL covering clinically relevant concentrations of fibrin split products. The protocol uses 50 uL of serum or plasma per well, with incubation steps performed at room temperature and a total run time of roughly 3.5 hours. The capture antibody is human-specific, showing no significant cross-reactivity with intact fibrinogen, D-dimer, or related fibrin degradation fragments, which makes the kit useful for distinguishing primary fibrinolysis from secondary fibrinolytic processes driven by thrombosis.

Storage requires the kit to be refrigerated at 4 degrees C, with reconstituted standards stored frozen at -20 degrees C in single-use aliquots to limit freeze-thaw cycles. The microplate format supports high-throughput screening in clinical research laboratories investigating thrombotic and hemostatic disorders, while the colorimetric endpoint is read on standard microplate readers fitted with a 450 nm filter. Assay Genie provides a detailed protocol booklet and dedicated technical support, allowing laboratories to integrate FDP quantification directly into coagulation research and biomarker discovery pipelines.