Human FDFT1/Farnesyl Diphosphate Farnesyltransferase 1 ELISA Kit

FDFT1, also known as squalene synthase, catalyzes the first committed step of sterol biosynthesis by joining two farnesyl pyrophosphate molecules to form squalene. This Assay Genie ELISA kit provides a quantitative plate-based immunoassay for measuring FDFT1 protein levels in human research samples, supporting studies of cholesterol biosynthesis, the mevalonate pathway, and lipid metabolism in academic and pharmaceutical settings.

The assay uses a two-antibody capture configuration with a chromogenic substrate for absorbance readout. Each microplate is supplied pre-coated and is sufficient for 96 individual determinations. Standard, sample, and control wells are processed in parallel, and results are interpolated against a recombinant human FDFT1 standard curve to yield concentration values in ng/mL.

Intended for research use only, the kit is applicable to studies of cardiovascular disease, hepatic lipid handling, statin and squalene synthase inhibitor pharmacology, and oncological contexts where cholesterol metabolism is altered. Compatible sample types include serum or plasma specimens, cell lysates, and tissue homogenates prepared from human sources. Researchers investigating perturbations of the mevalonate pathway, lipid-lowering drug responses, or squalene synthase expression in tumor models will find this assay useful for tracking protein-level changes across experimental conditions.