Human FBXW2 ELISA Kit (HUFI04306)

The FBXW2 protein (F-box and WD repeat domain containing 2) serves as the substrate-recognition component of an SCF-type E3 ubiquitin ligase complex, marking target proteins for ubiquitin-dependent proteasomal turnover. This Assay Genie kit enables investigators to quantify endogenous FBXW2 concentrations in human-derived liquid specimens, supporting research on Wnt/β-catenin pathway regulation, cell cycle control, and tumor biology where FBXW2-driven degradation of β-catenin and related substrates is biologically relevant.

Supplied as a complete kit with pre-coated microplate wells, capture and detection antibodies, dilution buffers, and substrate reagents, the protocol accommodates 100 µL of specimen per well and is intended for research use only. Published applications include expression profiling of FBXW2 in cervical and lung carcinoma models, where altered FBXW2 abundance has been linked to proliferation and invasive behavior, as well as mechanistic studies of substrate turnover within SCF ligase complexes and investigations of F-box protein contributions to oncogenic signaling networks.