Human FBXW11 ELISA Kit (HUFI04304)

The Assay Genie Human FBXW11 ELISA Kit enables quantitative measurement of F-box and WD repeat domain containing 11 (β-TrCP2), a substrate-recognition adaptor of the SCF ubiquitin ligase complex involved in NF-κB and Wnt signaling pathways. The assay supports investigations into protein turnover, cell cycle progression, and apoptosis in human-derived samples for translational research.

Each kit provides a pre-coated microplate, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, wash buffer, and TMB substrate solution, along with a complete step-by-step protocol. The procedure requires 50 µL of sample per well, with all reagents supplied ready-to-use or in concentrated form as indicated on the individual vial labels.

Store unopened components refrigerated between uses and protect from light; keep the substrate in its original amber bottle. Shelf life extends six months from the date of shipment when handled correctly, and reagents should be allowed to reach room temperature before opening. Intended for research use only in laboratory applications such as oncology, immunology, and signal transduction studies.