Human FBXO2 ELISA Kit (HUFI04310)

The FBXO2 protein (F-box only protein 2) functions as a substrate recognition subunit of the SCF E3 ubiquitin ligase complex, targeting N-glycosylated substrates for proteasomal degradation. Researchers studying neurodegeneration, particularly Alzheimer's disease pathways involving APP and BACE1 processing, frequently use this Assay Genie immunoassay. The microplate format supports quantitative measurement of human FBXO2 across a standard curve, with each kit providing the necessary pre-coated plate, assay buffers, and detection reagents. Reconstituted standards and prepared samples (100 µL per well) are incubated at room temperature before stop solution is added and absorbance is read. Applications include studies in cell biology, neuroscience, and oncology where FBXO2-mediated ubiquitination is under investigation, including glycoprotein quality control and substrate turnover research.