Human FBXL18 ELISA Kit (HUFI04315)

FBXL18 is an F-box protein that functions as a substrate-recognition subunit within the SCF E3 ubiquitin ligase complex, where it helps tag target proteins for proteasomal degradation. This quantitative immunoassay from Assay Genie is configured to measure FBXL18 in human specimens, supporting research into protein turnover, post-translational regulation, and pathways where ubiquitin-mediated destruction of cellular substrates plays a role. Investigators studying cell cycle control, immune signaling, or oncogenic transformation in which F-box proteins have been implicated can apply the kit to quantify FBXL18 levels across experimental cohorts.

The assay ships as a complete kit in a 96 Assays microplate arrangement and accepts human serum or plasma samples. Reagents should be kept refrigerated until first use, with working dilutions prepared on the day of the run. The plate-based readout allows quantification on a standard absorbance reader equipped with the appropriate filter. Running standards and unknowns in duplicate or replicate is recommended for reliable curve fitting and inter-well reproducibility.

Kit components generally include a pre-coated microwell plate, biotinylated detection reagent, enzyme conjugate, chromogenic substrate, stop solution, concentrated wash buffer, and a recombinant FBXL18 calibrator suitable for generating a multi-point standard curve. Each manufactured lot is quality-checked by Assay Genie for intra-assay and inter-assay precision.