Human FBN3/Fibrillin 3 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit uses a double antibody method for the quantitative determination of human FBN3 (Fibrillin-3) in research samples. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, with reactivity confirmed in human specimens.

Reported performance specifications include a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Spiking studies report serum recovery of 92–102% with an average of 96%, EDTA plasma recovery of 88–99% (average 95%), and heparin plasma recovery of 89–101% (average 95%). Linearity in serum across 1:2, 1:4, and 1:8 dilutions spans 86–101%, 87–103%, and 85–104% respectively. Unopened components are stored at 2–8°C for up to 12 months. The associated UniProt entry is Q75N90, with listed synonyms including FBN3, Fibrillin 3, and Fibrillin-3.

Fibrillin-3 contributes to the structural integrity of connective tissues as an extracellular matrix component, and abnormalities in the protein have been linked to conditions such as Marfan syndrome, supporting research applications in connective tissue disorders.