Human Fatty Acid Synthase/FASN ELISA Kit

From Assay Genie, this Human Fatty Acid Synthase/FASN ELISA Kit is a sandwich ELISA using a double-antibody configuration designed for quantitative detection of FASN in human samples, with colorimetric readout via TMB substrate and absorbance measurement at 450 nm.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a reported sensitivity of 0.188 ng/mL and a measurable range of 0.313–20 ng/mL. Linearity in serum (n = 5) at 1:2, 1:4, and 1:8 dilutions spans 88–99%, 92–101%, and 85–97%; EDTA plasma spans 91–104%, 85–101%, and 88–98%; heparin plasma spans 91–103%, 85–100%, and 81–99%. Recovery averages 99% (range 92–104%) in serum, 95% (range 89–102%) in EDTA plasma, and 98% (range 90–105%) in heparin plasma.

The procedure involves equilibrating reagents, loading standards and samples for a 37 °C primary incubation of 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP–streptavidin binding for 30 minutes, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stopping. The associated Uniprot entry is P49327; listed synonyms are FASN, FAS, OA-519, and SDR27X1. The kit is intended for metabolism research and should be stored at 2–8 °C for up to 12 months.