Human Fatty acid desaturase 3 (FADS3) ELISA Kit

Designed for the precise quantification of Human Fatty acid desaturase 3 (FADS3) in human research samples, this Sandwich ELISA Kit from Assay Genie uses a colorimetric detection method. It is reactive with human analytes and validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

FADS3 is an enzyme that participates in the biosynthesis of polyunsaturated fatty acids, and dysregulation of FADS3 has been linked to cardiovascular, metabolic, and inflammatory conditions. Also referred to as Delta(13) fatty acid desaturase, Delta(13) desaturase, and CYB5RP, the target localises to the endoplasmic reticulum membrane. The assay reports a detection range of 0.78-50 ng/mL and a sensitivity of 0.425 ng/mL, with intra-assay CV of 4.6% and inter-assay CV of 7.3%. Specificity is established against natural and recombinant human Fatty acid desaturase 3.

Linearity and recovery have been characterised across serum, EDTA plasma, and heparin plasma matrices, with serum recovery averaging 96% (range 90-102%) and plasma averaging 98% (range 92-104%). The product is intended for research use only, with exact storage details provided with the kit components.