Human FasL PharmaGenie ELISA Kit (SBRS0558)

Apoptosis and immunology laboratories use this Assay Genie microplate-format ELISA Kit to quantify soluble human Fas Ligand (FasL/CD178/TNFSF6), the type II transmembrane TNF-family ligand that engages Fas (CD95) to trigger caspase-mediated cell death. The kit is built around a capture-and-detection antibody configuration on a microplate pre-coated with anti-FasL capture reagent, making it well-suited to workflows that track FasL release during activation-induced cell death in lymphocyte cultures, immune-privilege studies, transplant rejection research, and tumor immune-evasion investigations.

Samples typically include cell culture supernatant from activated T-cell or NK-cell cultures, along with serum/plasma-type research specimens collected for translational studies. Standards and unknown samples are incubated alongside a biotinylated detection antibody, followed by streptavidin-HRP, chromogenic substrate, and stop solution. The resulting colorimetric readout is captured on a standard plate reader, with absorbance values back-calculated against the standard curve to yield FasL concentrations.

Supplied as a complete research-use-only kit, the package contains the pre-coated microplate, lyophilized recombinant FasL standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash and dilution buffers, chromogenic substrate, stop reagent, plate sealer, and a detailed protocol booklet. For research use only; not intended for diagnostic procedures.