Human Fascin (FSCN) ELISA Kit

Each kit from Assay Genie is supplied with a pre-coated strip plate, lyophilized recombinant FSCN1 reference standards, biotin-conjugated detection antibody, streptavidin-HRP working solution, TMB substrate, stop reagent, and concentrated wash buffer sufficient for the full protocol run. The capture antibody is pre-immobilized on the plate, so researchers add standards and diluted samples directly, followed sequentially by the detection reagent and enzyme conjugate; color development with TMB is then read at the appropriate wavelength after stop solution is added. Standard curves are generated from a seven-point serial dilution series, and intra-assay precision is documented in the lot-specific certificate of analysis supplied with the kit. Researchers studying metastatic breast, colorectal, and head-and-neck carcinomas commonly pair this assay with western blot validation of fascin upregulation, while cytoskeletal biologists use it to quantify changes in FSCN1 expression during invadopodia formation in cell migration assays. Reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles, and the working detection antibody should be kept refrigerated and used within the working day for best performance.