Human Fas PharmaGenie ELISA Kit (SBRS0557)

The Human Fas protein, also called CD95 or APO-1, is a 48 kDa transmembrane death receptor belonging to the tumor necrosis factor receptor superfamily. Engagement of Fas by its cognate ligand (FasL/CD178) triggers the extrinsic apoptotic cascade via FADD and caspase-8 activation, leading to programmed cell death. Soluble Fas (sFas) isoforms generated through alternative mRNA splicing circulate systemically and can antagonize receptor-mediated apoptosis, linking dysregulated Fas signaling to autoimmune lymphoproliferative syndrome, systemic lupus erythematosus, leukemias, and tumor immune evasion.

This Assay Genie kit delivers quantitative measurement of Fas concentrations in cell culture supernatants, plasma, serum, cell lysates, and tissue lysates, supporting apoptosis research, oncology investigations, immunological studies, and biomarker screening. Each kit includes a pre-coated microplate strip plate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, and wash buffer. Researchers typically require 100 µL of sample per well and should complete the ELISA within the kit's stated shelf life. Unopened kits are kept refrigerated, while reconstituted components are stored frozen at -20 °C and protected from light.