Human FARSA ELISA Kit (HUFI04327)

FARSA (Phenylalanyl-tRNA Synthetase Subunit Alpha) is an aminoacyl-tRNA synthetase enzyme that catalyzes the attachment of phenylalanine to its cognate tRNA during protein biosynthesis. This quantitative immunoassay from Assay Genie enables researchers to measure human FARSA protein levels, supporting studies in enzyme biology, translational regulation, and investigations of aminoacyl-tRNA synthetase dysregulation in cancer, autoimmune disorders, and metabolic disease. The kit provides reagents for use in cell lysate analysis, tissue homogenate profiling, and characterization of phenylalanyl-tRNA synthetase activity across experimental conditions. Each microplate is shipped in a sealed pouch with desiccant and includes capture antibody-coated wells, recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. Researchers add samples and reagents to the wells, incubate, and measure absorbance to quantify FARSA concentration. The kit format supports 96 determinations per plate, providing sufficient throughput for screening studies. Documentation includes a detailed protocol with recommended dilutions, expected standard curve performance, and troubleshooting guidance to help optimize signal-to-noise ratios. This ELISA is intended for research use only and is validated for reactivity with human samples, making it a useful tool for laboratories studying translation-related enzymes and their role in disease mechanisms.