Human FAP/Fibroblast activation protein alpha ELISA Kit

Assay Genie offers a Sandwich ELISA (Double Antibody) kit for quantitative detection of human Fibroblast Activation Protein Alpha (FAP) in research samples. The assay is described as a reliable, accurate tool with high sensitivity and specificity, supporting studies in tissue remodeling and cancer biology, where its overexpression has been linked to multiple tumour types and makes it a useful biomarker of the tumour microenvironment.

Reported performance includes a sensitivity of 0.375 ng/mL and a working range of 0.625–40 ng/mL, with validated use on serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. All reagents are stored at 2-8°C for 12 months. The workflow uses a biotin-labelled detection antibody, HRP-Streptavidin (SABC) and TMB substrate, with absorbance read at 450 nm after a stop step.

Reported linearity at 1:2, 1:4 and 1:8 spans 86–101% (serum), 87–105% (EDTA plasma) and 87–99% (heparin plasma), and recovery averages of 93% (serum), 93% (EDTA plasma) and 95% (heparin plasma) are given across n=5 samples.