Human Family With Sequence Similarity 20, Member A (FAM20A) ELISA Kit

FAM20A is a Golgi-localized member of the FAM20 family of secretory pathway proteins, whose dysfunction is linked to Amelogenesis Imperfecta and Enamel Renal Syndrome, conditions characterized by defective enamel mineralization and gingival fibromatosis. This quantitative assay from Assay Genie enables researchers to measure FAM20A concentrations in biological samples for studies of biomineralization, tooth development, and mineralized tissue biology.

The kit uses a microplate format with all reagents supplied ready for use. Reconstituted standards should be kept refrigerated at 4°C for short-term use, while the remaining components are best stored frozen at -20°C to preserve activity through repeated freeze-thaw cycles. Researchers working on dental enamel pathology, osteogenesis, or mineralization disorders will find this assay useful for quantifying FAM20A levels, with results that pair well with downstream applications such as western blot validation, immunohistochemistry of mineralized tissue sections, and PCR-based gene expression analysis.

Each kit contains a pre-coated plate, biotinylated detection antibody, standards, and substrate. The assay accommodates a 100 uL sample volume per well, with intra-assay and inter-assay reproducibility established across a defined working range.