Human FAM3C (Family With Sequence Similarity 3 Member C) ELISA Kit

This Sandwich ELISA Kit from Assay Genie quantifies human FAM3C (also referenced as ILEI, Interleukin-like EMT Inducer), a secreted cytokine-like protein implicated in epithelial-mesenchymal transition and tumor metastasis pathways. Researchers use the kit to measure circulating FAM3C levels in serum or plasma samples, supporting oncology, inflammation, and biomarker discovery studies.

The assay is built on a pre-coated 96-well microplate and employs a colorimetric readout, typically measured at 450 nm, with a standard curve generated from recombinant human FAM3C. Reconstituted standards and biotinylated detection antibody are commonly stored at -20 °C, while the microplate and wash buffer are kept refrigerated at 2-8 °C. Each kit provides sufficient reagents for one full 96-well plate, accommodating blank wells and a standard dilution series.

Intended for research use only, the workflow accommodates typical sample volumes of 50-100 µL per well, with intra- and inter-assay precision commonly reported under 10% CV when protocol directions are followed. The kit can be applied to cell culture supernatant, tissue homogenate, and biological fluid analyses beyond the validated serum and plasma matrices.